Does chloroquine increase or decrease cell viability

Discussion in 'Canadian Drug' started by Tiratron, 12-Mar-2020.

  1. DPZ Moderator

    Does chloroquine increase or decrease cell viability


    Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it.

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    Confirm that viral inhibition is independent of chloroquine cytotoxicity, cell viability was analyzed in uninfected cells treated with chloroquine 1.56 to 200 µM for 5 days. Chloroquine does not impact cell viability at 50 µM or lower concentrations Fig. 1D. We further analyzed whether chloroquine treatment could protect Vero cells from ZIKV- Commercially available automated cell counters based on trypan blue dye-exclusion are widely used in industrial cell culture process development and manufacturing to increase throughput and eliminate inherent variability in subjective interpretation associated with manual hemocytometers. When using these cell counters, sample dilution is often necessary to stay within the assay measurement. Of autophagy by chloroquine may increase cells sensitivity to FTS. In order to examine the contribution of active Ras to the effect of the combined treatment on cell viability, we have used Rat-1 fibroblasts and Rat-1 transfected with H-Ras 12V, known as EJ cells. As shown in Figure 2C, Ras transformed, EJ cells responded to FTS

    The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations. In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead.

    Does chloroquine increase or decrease cell viability

    Chloroquine Uses, Side Effects & Warnings -, Effects of diluents on cell culture viability measured by.

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  7. There were no significant changes in the cell viability after exposure to chloroquine for 24 h. However, exposure to 100 nM but not 10 nM bafilomycin, decreased cell viability by approximately 35% Fig. 1 E.

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    Blots in Figure 6 show marked increase of LAMP-1 in chloroquine-treated cells, especially at 10 μg/ml. LAMP-2 shows a similar, but less drastic, upregulation trend. Exogenous rhodamine-labeled dextran was applied to the chloroquine-treated cells. We observe a striking decrease in intracellular dextran in chloroquine-treated cells Figure 7A. PBMC cultures grown in the presence of 12.5 μM chloroquine for 7 days displayed a trend to decreased cell viability, compared with control cultures incubated in the absence of chloroquine median decrease 25%; first quartile 1%; third quartile 37.5%. The difference did not reach statistical significance Wilcoxon test P = 0.15. To. Chloroquine suppresses glioma cell viability and sensitizes radioresistant stem-like glioma cells to ionizing radiation and chemotherapy Conference Paper PDF Available in Neuro-Oncology.

     
  8. KarinaFOX New Member

    The resource you are looking for (or one of its dependencies) could have been removed, had its name changed, or is temporarily unavailable. Will you have Photosensitivity reaction with Plaquenil - eHealthMe Plaquenil Hydroxychloroquine - Side Effects, Dosage. Plaquenil Uses, Dosage & Side Effects -
     
  9. Hydroxychloroquine DermNet NZ Haemolysis in patients with glucose-6-phosphate dehydrogenase G6PD deficiency, aplastic anaemia, and leukopenia has been reported. The most extensive study to date evaluating G6PD deficiency with concurrent use of hydroxychloroquine reported no episodes of haemolysis in over 700 months of exposure among the 11 studied patients with G6PD.

    How does Plaquenil affect person with G6PD Deficiency?